Reporter

Part:BBa_K4799600:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtRCD1_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 26
    Illegal XbaI site found at 450
    Illegal SpeI site found at 1535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3344
    Illegal SpeI site found at 1535
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
    Illegal BglII site found at 1326
    Illegal BamHI site found at 1309
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 26
    Illegal XbaI site found at 450
    Illegal SpeI site found at 1535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 26
    Illegal XbaI site found at 450
    Illegal SpeI site found at 1535
    Illegal NgoMIV site found at 1759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1559
    Illegal BsaI.rc site found at 2174
    Illegal SapI.rc site found at 2345


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.


Source

plasmid

References